[BIC-announce] Seminar in Biomedical Engineering - Wednesday April 30th - 1h pm - Room 333

[Christophe Grova]

Dear all,

We will have our next  Biomedical Engineering seminar today

Wednesday  –  April 30th,  at 1 pm

Location: Room  333 Lyman Duff Building (Biomedical Engineering Dpt, 3775  University Street).

Speaker:  Prof. Amine Kamen PhD, Department of Bioengineering, McGill University

Dr. Kamen is candidate for associate membership in Biomedical Engineering department, so attendance from our staff members and their students is highly encouraged.


Title:
"Efficient production of viral vectors for gene delivery and vaccination"


Abstract:

HEK-293 cell lines have been widely used for over 30 years by the scientific community. Currently HEK-293 cells are the most efficient system for improved production of r-proteins and viral vector by large-scale transfection of suspension-growing cells in serum-free medium. Also, it is the most established cell line for the production of adeno- and adeno-associated viruses, retro-and lentiviruses for gene therapy application. Additionally HEK 293 cells sustain replication of many viruses that are evaluated as vaccines or viro-therapeutic agents. Consequently numerous viral vectors produced in HEK-293 cells have been approved for phase II and phase III clinical trials.

In this presentation, we will discuss achievements in process developments to support the large scale manufacturing of viral vectors and vaccines. On-going efforts include characterizing the metabolism of HEK-293 cells in culture, design of efficient operating and feeding strategies, and the development of advanced monitoring tools. Beside viral vectors for gene delivery, recent data demonstrating the suitability of the HEK-293 cells for manufacturing vectored vaccines and influenza vaccine will be presented. Through different examples, the presentation will also discuss practises and experiences in supporting translational research.



Key references:

Chahal, P.S., Schulze, E., Tran, R., Montes, J., and Kamen,* A.A. (2014) Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery. Journal of Virological Methods 196:163-173

Emma, P. and Kamen, A. (2013) Real-time monitoring of influenza virus production kinetics in HEK293 cell cultures. Biotechnology Progress 29(1): 275-284



Shen, C.F., Lanthier, S., Jacob, D., Montes, J., Beath, A., Beresford, A., and Kamen*, A. (2012) Process optimization and scale-up for production of rabies vaccine live adenovirus vector (AdRG1.3). Vaccine 30(2): 300-306.



Ansorge S., Lanthier S., Transfiguracion J., Durocher Y., Olivier Henry O. and Kamen A.(2009) Development of a Scalable Process for High  Yield Lentiviral Vector Production by Transient Transfection of HEK293 Suspension Cultures” Journal of Gene Medicine 11(10):868-76.



A list of upcoming seminars can be found at : http://www.mcgill.ca/bme/news/seminars

See you there


Christophe Grova

***************************
Christophe Grova, PhD
Assistant Professor
Biomedical Engineering Dpt
Neurology and Neurosurgery Dpt

Multimodal Functional Imaging Lab (Multi FunkIm)
Montreal Neurological Institute
Centre de Recherches en Mathématiques

Biomedical Engineering Department - Room 304
McGill University
3775 University Street, Montreal, Quebec, Canada,
H3A 2B4
email : christophe.grova at mcgill.ca<mailto:christophe.grova at mcgill.ca>
tel : (514) 398 2516
fax : (514) 398 7461

Web:
http://www.bic.mni.mcgill.ca/ResearchLabsMFIL/PeopleChristophe
http://www.bmed.mcgill.ca/
MultiFunkIm Lab:
http://www.bic.mni.mcgill.ca/ResearchLabsMFIL/HomePage

***************************



[X]
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://www.bic.mni.mcgill.ca/pipermail/bic-announce/attachments/20140428/8c27daa5/attachment-0001.html>