[BIC-announce] Seminar in Biomedical Engineering - Wednesday April 10th - 1 pm - Room 333

[Christophe Grova]

Dear all,

Our next Biomedical Engineering Dpt seminar  is today

Wednesday - April 10th,  at 1 pm

Location: Room  333 Lyman Duff Building (Biomedical Engineering Dpt, 3775  University Street).

Speaker: Grant Ongo, Master student under the supervision of Dr D. Juncker PhD, BME, McGill University

Since Grant is a Master student registered to the course BMDE500 who is giving this seminar as part of McGill conditions to obtain his M.Sc., special attendance from our department (staff and students) is really expected, in order to give Grant feedback on his research studies.

Title : Rapid, Low-Cost Detection of Pathogenic Bacteria for Point-of-Care Diagnostics

Abstract:
Hospital acquired bacterial infections are a growing problem in Canada and worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) is currently one of the most problematic North American "superbugs". Infected patients are at risk of blood stream infections following surgery, leading to high morbidity and mortality rates. Current methods of diagnosing MRSA carriers involve swabbing the nasal cavity, culturing the sample, and detecting by agar plating or PCR. These methods, while sensitive and accurate, are time consuming (1-2 days) and have limited throughput. We are developing a rapid, low-cost, microfluidic sensor that can be used at the point-of-care to detect antibiotic resistant bacteria, specifically MRSA. I will present results from my masters research on developing a novel, proof of concept immunoassay to detect whole bacteria cells using E. coli K-12 and O157:H7 (pathogenic). Bacteriophage and antibodies are used to capture live bacteria cells. Using a biotinylated detection antibody and streptavidin conjugated to gold nanoparticles, a silver coating is catalyzed on bacteria cells using silver enhancement reagents. This allows individual bacteria to become easily detectable via dark-field microscopy. This is advantageous since there is potential to reduce or eliminate the need for pre-culturing samples, and allows single bacteria to be counted for improved sensitivity. Future work will entail improving sensitivity by means of increasing surface area interactions using microbeads, and integrating the assay into our autonomous microfluidic circuits. In the future, this sensor may reduce the spread hospital acquired infections through rapid, high throughput screening of patients.


A list of upcoming seminars can be found at : http://www.mcgill.ca/bme/news/seminars

See you there


Christophe Grova


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Christophe Grova, PhD
Assistant Professor
Biomedical Engineering Dpt
Neurology and Neurosurgery Dpt

Montreal Neurological Institute
Centre de Recherches Mathématiques

Biomedical Engineering Department - Room 304
McGill University
3775 University Street, Montreal, Quebec, Canada, H3A 2B4
email : christophe.grova at mcgill.ca
tel : (514) 398 2516
fax : (514) 398 7461

web:
http://www.mni.mcgill.ca/research/gotman/members/christophe.html
http://www.bmed.mcgill.ca/
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